Nomenclature

Gene Symbol
SF3B2 (H. sapiens)
Other Gene Symbols
Cus1, SAP145, SF3B145, SF3b1, SF3b150
Full Name
splicing factor 3b, subunit 2, 145kDa
Other Names
SF3b145, SAP 145, pre-mRNA splicing factor SF3b 145 kDa subunit, pre-mRNA-splicing factor SF3b 145 kDa subunit, spliceosome associated protein 145, spliceosome-associated protein 145

Other Resources

Entrez: Gene Info
10992
Uniprot: Protein Info
SF3B2_HUMAN
Organism-Specific Database
—
BioGrid: Interaction Info
116188
PDB: Structures
2DO5
SpliProt3D: Structural Model
SF3B2

Gene Product Info

Molecular Weight (kD)
100.2
Domains
SAP
Motifs
—
Features
—
Complex Association
17S U2 snRNP, A complex, B complex, Bact complex, C complex, P complex
Class / Family
17S U2 snRNP
Notes
—

Orthologs

Name Organism
CUS1 S. cerevisiae
sap145 S. pombe
CG3605 D. melanogaster
W03F9.10 C. elegans

View orthologs at Homologene

Interactions
(from Hegele et al, 2012)

Interacting Partner 1 Interacting Partner 2 Interaction Type Score PubMed ID
SF3B2 LSM2 Y2H: Prey/Bait 0.8 22365833
SF3B2 LSM3 Y2H: Prey/Bait 1 22365833
SF3B2 SF3B4 Dervied from Literature — 7958871, 10490618, 16189514
SF3B2 SF3B4 Pull-down — 22365833
SF3B2 SF3B4 Y2H: Bidirectional 1 22365833
SF3B2 U2AF1 Y2H: Prey/Bait 0.5 22365833
SF3B2 U2AF1 Pull-down — 22365833
SF3B2 PRPF40A Y2H: Prey/Bait 1 22365833
SF3B2 SNRNP200 Y2H: Prey/Bait 0.4 22365833
SF3B2 PRPF8 Y2H: Prey/Bait 1 22365833
SF3B2 PRPF8 Pull-down — 22365833
SF3B2 PRPF3 Y2H: Prey/Bait 1 22365833
SF3B2 PRPF3 Pull-down — 22365833
SF3B2 XAB2 Y2H: Prey/Bait 0.5 22365833
SF3B2 XAB2 Pull-down — 22365833
SF3B2 DHX16 Y2H: Prey/Bait 0.62 22365833
SF3B2 DHX16 Pull-down — 22365833
SF3B2 ZNF830 Y2H: Prey/Bait 0.75 22365833
SF3B2 ZNF830 Pull-down — 22365833
SF3B2 RNF113A Y2H: Prey/Bait 0.58 22365833
SF3B2 RNF113A Pull-down — 22365833
SF3B2 SLU7 Y2H: Prey/Bait 0.55 22365833
SF3B2 SLU7 Pull-down — 22365833
SF3B2 RBM8A Y2H: Prey/Bait 0.5 22365833
SF3B2 THOC1 Y2H: Prey/Bait 0.62 22365833
SF3B2 RBM7 Dervied from Literature — 12634307
SF3B2 RBM7 Y2H: Prey/Bait 1 22365833
SF3B2 PCBP2 Y2H: Prey/Bait 0.27 22365833
SF3B2 HNRNPC Y2H: Prey/Bait 0.81 22365833
SF3B2 TTC14 Y2H: Prey/Bait 0.5 22365833
SF3B2 TOE1 Y2H: Prey/Bait 0.67 22365833
SF3B2 ZCCHC10 Y2H: Prey/Bait 1 22365833
SF3B2 FRA10AC1 Pull-down — 22365833
SF3B2 FRA10AC1 Y2H: Bidirectional 1 22365833

view interactions at SSPPIR...

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
B complex Sample#3, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 299 27377154
Pre-B complex Sample#3, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 205 27377154
17S U2 snRNP, H. sapiens, (Agafonov et al 2011) H. sapiens 153 21536652
Bact complex on PM5-10, H. sapiens, (Bessonov et al 2010) H. sapiens 136 20980672
Bact complex on PM5-20, H. sapiens, (Bessonov et al 2010) H. sapiens 118 20980672
Bact complex, deltaPrp2 on M3Actin, Orbitrap, 0.25 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 116 19935684
B complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 107 21536652
A complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 102 21536652
B complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 82 18322460
B complex Sample#2, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 80 27377154
Bact complex on PM5-20, H. sapiens, (Agafonov et al 2011) H. sapiens 76 21536652
Prp43 dissasociated ILS 11-12 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 74 23431055
ILS, released intron complex on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 72 23431055
Bact complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 71 19941820
B complex Sample#1, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 71 27377154
B complex via MS2 tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 70 16809785
B complex via MS2 tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 69 16809785
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 67 19935684
45SBact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 65 19935684
B complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 65 24448447
Pre-B complex Sample#2, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 65 27377154
Pre-B complex Sample#1, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 63 27377154
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 62 19935684
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 61 18322460
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 60 19935684
Prp43 dissasociated ILS 13-14 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 57 23431055
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 56 19935684
Prp43 dissasociated ILS 9-10 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 55 23431055
B complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 52 24448447
Prp19 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 49 21386897
45S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 49 19935684
Prp43 dissasociated ILS 7-8 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 48 23431055
B complex via tobramycin tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 46 16809785
Dre4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 46 21386897
B complex via tobramycin tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 44 16809785
B complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 42 19941820
Prp43 dissasociated ILS 15-16 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 39 23431055
A complex, H. sapiens, (Behzadnia et al 2007) H. sapiens 37 17332742
Prp43 dissasociated ILS 5-6 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 37 23431055
C complex on PM5, H. sapiens, (Bessonov et al 2010) H. sapiens 36 20980672
C complex on PM5, H. sapiens, (Agafonov et al 2011) H. sapiens 35 21536652
Bact complex, deltaPrp2 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 34 23431055
U2 snRNP IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 33 22110043
C complex on MINX-GG, H. sapiens, (Bessonov et al 2010) H. sapiens 31 20980672
Saf3 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 31 21386897
B complex on Ftz, sample 2, H. sapiens, (Herold et al 2009) H. sapiens 30 18981222
E complex IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 29 22110043
Post-catalytic complex, deltaPrp22 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 29 23431055
delta prp2 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 27 20089683
Bact complex, deltaPrp2 on M3Actin, Q-Tof, 1 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 27 19935684
P complex on AdML, short 3' exon, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 26 23345524
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 25 18981222
EDC-A complex Weri, H. sapiens, (Sharma et al 2008) H. sapiens 23 18193060
A-like complex (A') HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 22 18193060
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 22 18981222
E-like complex (E') Weri, H. sapiens, (Sharma et al 2008) H. sapiens 20 18193060
B complex on Ftz, sample 1, H. sapiens, (Herold et al 2009) H. sapiens 20 18981222
Urn1 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 19 21386897
Bact complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 17 19941820
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 17 18981222
C complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 16 18322460
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 15 12226669
E complex IP'd by antibody to U1A, H. sapiens, (Marakov et al 2011) H. sapiens 15 22110043
Prp43 dissasociated ILS 3-4 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 15 23431055
C complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 13 18322460
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 13 18981222
B complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 13 18981222
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 13 19935684
C complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 13 24448447
Saf2 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 12 21386897
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 12 23345524
Mixed complexes Weri, H. sapiens, (Sharma et al 2008) H. sapiens 11 18193060
EDC-A complex HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 11 18193060
C complex on AdML, maltose elute, H. sapiens, (Coltri et al 2011) H. sapiens 11 21526149
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 10 18322460
B complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 10 19941820
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 10 12226669
E-like complex (E') HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 10 18193060
C complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 10 18981222
C complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 10 24448447
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 9 17537823
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 9 18981222
C complex, H. sapiens, (Ilagan et al 2009) H. sapiens 8 19761775
C complex on ActinACAC, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 8 19941820
C complex, H. sapiens, (Jurica et al 2002) H. sapiens 8 11991638
EDC-E complex Weri, H. sapiens, (Sharma et al 2008) H. sapiens 8 18193060
C complex 1M NaCl-washed core, H. sapiens, (Bessonov et al 2008) H. sapiens 6 18322460
Released with ILS, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 5 23431055
U2/U5/U6 complex Prp19-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 4 11884590
C complex on ActinD31, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 4 19941820
EDC-E complex HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 4 18193060
SRM160 pulldown with BIC8 antibody, H. sapiens, (McCracken et al 2005) H. sapiens 4 16159877
SRM300 pulldown with Srm300 antibody, H. sapiens, (McCracken et al 2005) H. sapiens 4 16159877
C complex exons trimmed, H. sapiens, (Ilagan et al 2009) H. sapiens 3 19761775
penta-snRNP, S. cerevisiae, (Stevens et al 2002) S. cerevisiae 3 11804584
H complex, H. sapiens, (Jurica et al 2002) H. sapiens 3 11991638
U2/U5/U6 complex CDC5-tag, S. pombe, (Ohi et al 2002) S. pombe 2 11884590
SRM160 pulldown with Srm160 antibody, H. sapiens, (McCracken et al 2005) H. sapiens 2 16159877
Prp43 dissasociated ILS 1-2 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 2 23431055
U2/U5/U6 complex Snt309-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 1 11884590
SF3b, H. sapiens, (Will et al 2002) H. sapiens ■ 12234937
U2 17S, H. sapiens, (Will et al 2002) H. sapiens ■ 12234937
E complex Weri, H. sapiens, (Sharma et al 2005) H. sapiens ■ 16109373
U11/U12 18S di-snRNP, H. sapiens, (Will et al 2004) H. sapiens ■ 15146077
B* complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
H complex Weri, H. sapiens, (Sharma et al 2005) H. sapiens ■ 16109373
Mixed spliceosome complexes, H. sapiens, (Neubauer et al 1999) H. sapiens ■ 9731529
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931
CDC5L pulldown, H. sapiens, (Ajuh et al 1999) H. sapiens ■ 11101529
A complex, H. sapiens, (Hartmuth et al 2002) H. sapiens ■ 12477934
Prp1 tap-tag pulldown, S. pombe, (Carnahan et al 2005) S. pombe ■ 15755920
PSF-pulldown, without splicing substrate, H. sapiens, (Peng et al 2006) H. sapiens ■ 17114945
PSF-pulldown, with AdMl splicing subtrate, H. sapiens, (Peng et al 2006) H. sapiens ■ 17114945
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae ■ 19935684
B complex on MINX-MS2, ATPgammaS block, 40S glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562
A complex on MINX-MS2, ATPgammaS block, glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562

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