Nomenclature

Gene Symbol
SNRNP200 (H. sapiens)
Other Gene Symbols
ASCC3L1, BRR2, FLJ11521, HELIC2, RP33
Full Name
small nuclear ribonucleoprotein 200kDa (U5)
Other Names
U5-200kD, BRR2 homolog, U5 small nuclear ribonucleoprotein 200 kDa helicase, U5 snRNP-specific 200 kDa protein, activating signal cointegrator 1 complex subunit 3-like 1

Other Resources

Entrez: Gene Info
23020
Uniprot: Protein Info
U520_HUMAN
Organism-Specific Database
—
BioGrid: Interaction Info
116661
PDB: Structures
2Q0Z
SpliProt3D: Structural Model
ASCC3L1

Gene Product Info

Molecular Weight (kD)
244.5
Domains
Helicase ATP-binding, Helicase C-terminal, SEC63
Motifs
DEIH box, DEVH box
Features
—
Complex Association
B complex, Bact complex, C complex, P complex, tri-snRNP, U5 snRNP
Class / Family
U5 snRNP
Notes
—

Orthologs

Name Organism
BRR2 S. cerevisiae
brr2 S. pombe
l(3)72Ab D. melanogaster
Y46G5A.4 C. elegans

View orthologs at Homologene

Interactions
(from Hegele et al, 2012)

Interacting Partner 1 Interacting Partner 2 Interaction Type Score PubMed ID
SNRNP200 LSM8 Y2H: Bait/Prey 0.75 22365833
SNRNP200 SF3B2 Y2H: Bait/Prey 0.4 22365833
SNRNP200 SMNDC1 Dervied from Literature — 11331595
SNRNP200 SNRNP200 Y2H: Homodimer 1 22365833
SNRNP200 SNRNP200 Pull-down — 22365833
SNRNP200 SNRNP200 Pull-down — 22365833
SNRNP200 PRPF8 Dervied from Literature — 9774689, 16723661, 17317632, 19098916
SNRNP200 EFTUD2 Dervied from Literature — 16723661
SNRNP200 PRPF6 Dervied from Literature — 16723661
SNRNP200 SART1 Dervied from Literature — 16723661
SNRNP200 RNF113A Y2H: Prey/Bait 0.81 22365833
SNRNP200 RNF113A Pull-down — 22365833

view interactions at SSPPIR...

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
B complex Sample#3, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 1925 27377154
Pre-B complex Sample#3, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 1316 27377154
Bact complex on PM5-20, H. sapiens, (Bessonov et al 2010) H. sapiens 623 20980672
Bact complex, deltaPrp2 on M3Actin, Orbitrap, 0.25 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 548 19935684
Bact complex on PM5-10, H. sapiens, (Bessonov et al 2010) H. sapiens 542 20980672
B complex Sample#2, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 521 27377154
B complex Sample#1, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 519 27377154
C complex on MINX-GG, H. sapiens, (Bessonov et al 2010) H. sapiens 490 20980672
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 375 18322460
C complex on PM5, H. sapiens, (Bessonov et al 2010) H. sapiens 370 20980672
B complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 355 24448447
Pre-B complex Sample#1, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 343 27377154
C complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 334 18322460
C complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 329 18322460
Prp19 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 316 21386897
C complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 314 24448447
Pre-B complex Sample#2, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 307 27377154
45S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 284 19935684
ILS, released intron complex on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 275 23431055
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 268 19935684
B complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 267 18322460
B complex via MS2 tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 267 16809785
45SBact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 262 19935684
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 257 18322460
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 256 19935684
Bact complex, deltaPrp2 on M3Actin, Q-Tof, 1 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 245 19935684
Bact complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 237 19941820
B complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 226 24448447
Bact complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 197 19941820
B complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 197 19941820
Prp43 dissasociated ILS 7-8 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 174 23431055
C complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 171 18981222
Prp43 dissasociated ILS 9-10 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 168 23431055
Prp43 dissasociated ILS 11-12 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 168 23431055
Prp43 dissasociated ILS 5-6 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 162 23431055
C complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 155 24448447
tri-snRNP, H. sapiens, (Agafonov et al 2011) H. sapiens 153 21536652
Post-catalytic complex, deltaPrp22 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 143 23431055
Prp17tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 139 21386897
Bact complex on PM5-20, H. sapiens, (Agafonov et al 2011) H. sapiens 138 21536652
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 136 18981222
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 134 18981222
B complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 128 21536652
C complex on ActinACAC, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 126 19941820
B complex via tobramycin tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 124 16809785
B complex on Ftz, sample 2, H. sapiens, (Herold et al 2009) H. sapiens 120 18981222
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 119 19935684
C complex on PM5, H. sapiens, (Agafonov et al 2011) H. sapiens 116 21536652
Prp43 dissasociated ILS 3-4 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 115 23431055
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 113 18981222
Saf3 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 113 21386897
C complex on ActinD31, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 110 19941820
Bact complex, deltaPrp2 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 110 23431055
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 108 18981222
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 99 18981222
B complex via MS2 tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 95 16809785
P complex on AdML, short 3' exon, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 93 23345524
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 91 23345524
Prp43 dissasociated ILS 13-14 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 91 23431055
B complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 90 19941820
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 87 19935684
B complex on Ftz, sample 1, H. sapiens, (Herold et al 2009) H. sapiens 86 18981222
Cwf2 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 83 21386897
Prp43 dissasociated ILS 15-16 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 74 23431055
Dre4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 73 21386897
C complex 1M NaCl-washed core, H. sapiens, (Bessonov et al 2008) H. sapiens 66 18322460
C complex, H. sapiens, (Ilagan et al 2009) H. sapiens 63 19761775
delta prp2 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 56 20089683
C complex on AdML, 3M urea wash, H. sapiens, (Coltri et al 2011) H. sapiens 56 21526149
delta prp16 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 55 20089683
Prp43 dissasociated ILS 1-2 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 55 23431055
B complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 54 18981222
C complex, H. sapiens, (Jurica et al 2002) H. sapiens 50 11991638
SKIP GST-tag pulldown, H. sapiens, (Zhang et al 2003) H. sapiens 48 12840015
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 44 17537823
U2/U5/U6 complex Prp19-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 42 11884590
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 41 19935684
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 39 12226669
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 36 12226669
A complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 36 21536652
E complex IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 36 22110043
C complex exons trimmed, H. sapiens, (Ilagan et al 2009) H. sapiens 32 19761775
Urn1 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 31 21386897
penta-snRNP, S. cerevisiae, (Stevens et al 2002) S. cerevisiae 30 11804584
delta prp22 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 26 20089683
C complex on AdML, maltose elute, H. sapiens, (Coltri et al 2011) H. sapiens 18 21526149
U2/U5/U6 complex CDC5-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 17 11884590
B complex via tobramycin tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 17 16809785
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 17 19935684
U2/U5/U6 complex CDC5-tag, S. pombe, (Ohi et al 2002) S. pombe 15 11884590
Mixed complexes Weri, H. sapiens, (Sharma et al 2008) H. sapiens 11 18193060
Clf1p-pulldown, S. cerevisiae, (Wang et al 2003) S. cerevisiae 10 12509417
Aim4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 9 21386897
Prp8 tap-tag pulldown, S. cerevisiae, (Khanna et al 2009) S. cerevisiae 9 19789211
mRNP on AdML, H. sapiens, (Merz et al 2007) H. sapiens 7 17095540
U2/U5/U6 complex Snt309-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 6 11884590
H complex, H. sapiens, (Jurica et al 2002) H. sapiens 6 11991638
U2/U5/U6 complex Cwc1-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 3 11884590
H complex, H. sapiens, (Merz et al 2007) H. sapiens 3 17095540
U2 snRNP IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 3 22110043
mRNP on BetaGlobin, H. sapiens, (Merz et al 2007) H. sapiens 2 17095540
Released from Post-catalytic complex, deltaPrp22, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 2 23431055
U2/U5/U6 complex Cwc2-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 1 11884590
mRNP unspliced control, H. sapiens, (Merz et al 2007) H. sapiens 1 17095540
Cwc21 tap-tag pulldown, S. cerevisiae, (Khanna et al 2009) S. cerevisiae 1 19789211
tri-snRNP, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
tri-snRNP, S. cerevisiae, (Stevens et al 1999) S. cerevisiae ■ 11720284
U5 35S complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
tri-snRNP, S. cerevisiae, (Gottschalk et al 1999) S. cerevisiae ■ 10449419
B* complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931
A complex, H. sapiens, (Hartmuth et al 2002) H. sapiens ■ 12477934
Prp1 tap-tag pulldown, S. pombe, (Carnahan et al 2005) S. pombe ■ 15755920
PSF-pulldown, without splicing substrate, H. sapiens, (Peng et al 2006) H. sapiens ■ 17114945
PSF-pulldown, with AdMl splicing subtrate, H. sapiens, (Peng et al 2006) H. sapiens ■ 17114945
B complex on MINX-MS2, ATPgammaS block, 40S glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562

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