Nomenclature

Gene Symbol
EFTUD2 (H. sapiens)
Other Gene Symbols
DKFZp686E24196, FLJ44695, KIAA0031, MFDM, Snrp116
Full Name
elongation factor Tu GTP binding domain containing 2
Other Names
U5-116kD, 116 kDa U5 small nuclear ribonucleoprotein component, SNU114 homolog, U5 snRNP-specific protein, 116 kDa, elongation factor Tu GTP-binding domain-containing protein 2, hSNU114

Other Resources

Entrez: Gene Info
9343
Uniprot: Protein Info
U5S1_HUMAN
Organism-Specific Database
—
BioGrid: Interaction Info
114749
PDB: Structures
—
SpliProt3D: Structural Model
EFTUD2

Gene Product Info

Molecular Weight (kD)
109.4
Domains
—
Motifs
—
Features
—
Complex Association
B complex, Bact complex, C complex, P complex, tri-snRNP, U5 snRNP
Class / Family
U5 snRNP
Notes
—

Orthologs

Name Organism
SNU114 S. cerevisiae
cwf10 S. pombe
CG4849 D. melanogaster
ZK328.2 C. elegans
EFTUD2 G. gallus

View orthologs at Homologene

Interactions
(from Hegele et al, 2012)

Interacting Partner 1 Interacting Partner 2 Interaction Type Score PubMed ID
EFTUD2 LSM8 Y2H: Bait/Prey 0.52 22365833
EFTUD2 SF3B3 Y2H: Bidirectional 0.63 22365833
EFTUD2 SF3B4 Pull-down — 22365833
EFTUD2 SF3B4 Y2H: Prey/Bait 0.81 22365833
EFTUD2 SF1 Y2H: Prey/Bait 0.62 22365833
EFTUD2 SNRNP200 Dervied from Literature — 16723661
EFTUD2 PRPF8 Dervied from Literature — 9774689, 16723661, 17317632
EFTUD2 IK Pull-down — 22365833
EFTUD2 IK Y2H: Bidirectional 0.74 22365833
EFTUD2 HSPB1 Y2H: Prey/Bait 0.97 22365833
EFTUD2 HSPB1 Pull-down — 22365833

view interactions at SSPPIR...

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
Cwf2 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 1202 21386897
Prp19 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 1166 21386897
Prp17tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 796 21386897
B complex Sample#3, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 788 27377154
Pre-B complex Sample#3, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 545 27377154
Bact complex on PM5-20, H. sapiens, (Bessonov et al 2010) H. sapiens 354 20980672
Bact complex on PM5-10, H. sapiens, (Bessonov et al 2010) H. sapiens 335 20980672
C complex on MINX-GG, H. sapiens, (Bessonov et al 2010) H. sapiens 245 20980672
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 232 19935684
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 231 19935684
B complex Sample#2, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 228 27377154
Bact complex, deltaPrp2 on M3Actin, Orbitrap, 0.25 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 227 19935684
B complex Sample#1, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 223 27377154
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 222 19935684
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 217 19935684
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 215 19935684
C complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 212 18322460
45S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 206 19935684
C complex on PM5, H. sapiens, (Bessonov et al 2010) H. sapiens 198 20980672
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 195 19935684
45SBact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 195 19935684
B complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 169 18322460
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 168 18322460
Bact complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 159 19941820
B complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 159 24448447
B complex via MS2 tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 153 16809785
Bact complex on PM5-20, H. sapiens, (Agafonov et al 2011) H. sapiens 151 21536652
Pre-B complex Sample#2, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 150 27377154
Pre-B complex Sample#1, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 141 27377154
B complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 140 24448447
B complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 136 21536652
C complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 133 18322460
ILS, released intron complex on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 131 23431055
C complex on ActinACAC, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 130 19941820
C complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 128 24448447
C complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 127 24448447
C complex on PM5, H. sapiens, (Agafonov et al 2011) H. sapiens 121 21536652
tri-snRNP, H. sapiens, (Agafonov et al 2011) H. sapiens 116 21536652
C complex on ActinD31, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 113 19941820
Prp43 dissasociated ILS 7-8 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 109 23431055
C complex 1M NaCl-washed core, H. sapiens, (Bessonov et al 2008) H. sapiens 108 18322460
Bact complex, deltaPrp2 on M3Actin, Q-Tof, 1 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 97 19935684
Saf3 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 91 21386897
C complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 90 18981222
Prp43 dissasociated ILS 5-6 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 83 23431055
Dre4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 81 21386897
B complex via MS2 tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 76 16809785
Prp43 dissasociated ILS 11-12 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 73 23431055
Prp43 dissasociated ILS 9-10 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 69 23431055
B complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 66 19941820
P complex on AdML, short 3' exon, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 66 23345524
Post-catalytic complex, deltaPrp22 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 64 23431055
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 61 18981222
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 59 23345524
Bact complex, deltaPrp2 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 58 23431055
B complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 54 19941820
Bact complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 51 19941820
B complex via tobramycin tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 51 16809785
A complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 51 21536652
C complex, H. sapiens, (Ilagan et al 2009) H. sapiens 50 19761775
Prp43 dissasociated ILS 13-14 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 49 23431055
B complex on Ftz, sample 2, H. sapiens, (Herold et al 2009) H. sapiens 46 18981222
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 46 18981222
delta prp22 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 44 20089683
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 43 18981222
B complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 42 18981222
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 41 18981222
Prp43 dissasociated ILS 3-4 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 40 23431055
C complex exons trimmed, H. sapiens, (Ilagan et al 2009) H. sapiens 38 19761775
U2/U5/U6 complex CDC5-tag, S. pombe, (Ohi et al 2002) S. pombe 38 11884590
U2/U5/U6 complex Prp19-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 34 11884590
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 34 18981222
B complex on Ftz, sample 1, H. sapiens, (Herold et al 2009) H. sapiens 33 18981222
Urn1 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 32 21386897
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 31 18322460
Supraspliceosome, G. gallus, (Chen et al 2007) G. gallus 31 17537823
B complex via tobramycin tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 30 16809785
delta prp2 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 27 20089683
delta prp16 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 26 20089683
C complex, H. sapiens, (Jurica et al 2002) H. sapiens 26 11991638
U2/U5/U6 complex CDC5-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 24 11884590
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 22 12226669
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 22 17537823
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 19 12226669
Prp43 dissasociated ILS 1-2 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 19 23431055
penta-snRNP, S. cerevisiae, (Stevens et al 2002) S. cerevisiae 18 11804584
Saf1 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 15 21386897
E complex IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 13 22110043
C complex on AdML, 3M urea wash, H. sapiens, (Coltri et al 2011) H. sapiens 13 21526149
U2/U5/U6 complex Snt309-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 12 11884590
CACN-1 pulldown with FLAG-HA tandem affinity from C. elegans, C. elegans, (Doherty et al 2014) C. elegans 12 24948787
U2/U5/U6 complex Cwc2-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 11 11884590
SKIP GST-tag pulldown, H. sapiens, (Zhang et al 2003) H. sapiens 11 12840015
Mixed complexes Weri, H. sapiens, (Sharma et al 2008) H. sapiens 10 18193060
H complex, H. sapiens, (Jurica et al 2002) H. sapiens 10 11991638
Saf2 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 10 21386897
C complex on AdML, maltose elute, H. sapiens, (Coltri et al 2011) H. sapiens 10 21526149
U2/U5/U6 complex Cwc1-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 9 11884590
E complex IP'd by antibody to U1A, H. sapiens, (Marakov et al 2011) H. sapiens 8 22110043
mRNP on AdML, H. sapiens, (Merz et al 2007) H. sapiens 7 17095540
Clf1p-pulldown, S. cerevisiae, (Wang et al 2003) S. cerevisiae 5 12509417
U2 snRNP IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 4 22110043
SRM160 pulldown with BIC8 antibody, H. sapiens, (McCracken et al 2005) H. sapiens 4 16159877
mRNP on BetaGlobin, H. sapiens, (Merz et al 2007) H. sapiens 3 17095540
SRM300 pulldown with Srm300 antibody, H. sapiens, (McCracken et al 2005) H. sapiens 3 16159877
H complex, H. sapiens, (Merz et al 2007) H. sapiens 2 17095540
SRM160 pulldown with Srm160 antibody, H. sapiens, (McCracken et al 2005) H. sapiens 1 16159877
Prp43 dissasociated ILS 15-16 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 1 23431055
tri-snRNP, S. cerevisiae, (Stevens et al 1999) S. cerevisiae ■ 11720284
U5 35S complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
B* complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
tri-snRNP, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931
A complex, H. sapiens, (Hartmuth et al 2002) H. sapiens ■ 12477934
Prp1 tap-tag pulldown, S. pombe, (Carnahan et al 2005) S. pombe ■ 15755920
B complex on MINX-MS2, ATPgammaS block, 40S glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562

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