Nomenclature

Gene Symbol
CRNKL1 (H. sapiens)
Other Gene Symbols
CLF, CRN, Clf1, HCRN, MSTP021
Full Name
crooked neck pre-mRNA splicing factor-like 1 (Drosophila)
Other Names
CRN, Crn, crooked neck-like 1, crooked neck homolog, crooked neck-like 1, crooked neck-like protein 1

Other Resources

Entrez: Gene Info
51340
Uniprot: Protein Info
CRNL1_HUMAN
Organism-Specific Database
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BioGrid: Interaction Info
119487
PDB: Structures
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SpliProt3D: Structural Model
CRNKL1

Gene Product Info

Molecular Weight (kD)
100.5
Domains
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Motifs
—
Features
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Complex Association
B complex, Bact complex, C complex, P complex
Class / Family
PRP19 related
Notes
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Orthologs

Name Organism
CLF1 S. cerevisiae
cwf4 S. pombe
crn D. melanogaster
M03F8.3 C. elegans
CRNKL1 G. gallus

View orthologs at Homologene

Interactions
(from Hegele et al, 2012)

Interacting Partner 1 Interacting Partner 2 Interaction Type Score PubMed ID
CRNKL1 CCDC12 Y2H: Prey/Bait 0.62 22365833
CRNKL1 CCDC12 Pull-down — 22365833
CRNKL1 PPIL2 Dervied from Literature — 15189447

view interactions at SSPPIR...

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
Prp19 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 810 21386897
Cwf2 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 784 21386897
Prp17tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 731 21386897
Bact complex on PM5-20, H. sapiens, (Bessonov et al 2010) H. sapiens 263 20980672
Bact complex on PM5-10, H. sapiens, (Bessonov et al 2010) H. sapiens 186 20980672
C complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 176 18322460
C complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 174 24448447
Bact complex on PM5-20, H. sapiens, (Agafonov et al 2011) H. sapiens 169 21536652
C complex on PM5, H. sapiens, (Agafonov et al 2011) H. sapiens 153 21536652
C complex on PM5, H. sapiens, (Bessonov et al 2010) H. sapiens 150 20980672
C complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 143 18322460
C complex on MINX-GG, H. sapiens, (Bessonov et al 2010) H. sapiens 126 20980672
ILS, released intron complex on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 125 23431055
C complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 112 24448447
Bact complex, deltaPrp2 on M3Actin, Orbitrap, 0.25 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 111 19935684
B complex Sample#3, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 107 27377154
C complex 1M NaCl-washed core, H. sapiens, (Bessonov et al 2008) H. sapiens 102 18322460
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 93 19935684
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 90 19935684
C complex, H. sapiens, (Ilagan et al 2009) H. sapiens 89 19761775
45S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 89 19935684
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 88 19935684
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 86 18322460
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 86 19935684
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 80 19935684
B complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 80 24448447
45SBact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 73 19935684
B complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 66 18322460
C complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 62 18981222
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 59 19935684
Prp43 dissasociated ILS 7-8 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 58 23431055
Bact complex, deltaPrp2 on M3Actin, Q-Tof, 1 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 55 19935684
B complex Sample#1, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 55 27377154
Prp43 dissasociated ILS 9-10 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 49 23431055
C complex on ActinD31, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 44 19941820
C complex exons trimmed, H. sapiens, (Ilagan et al 2009) H. sapiens 44 19761775
B complex via MS2 tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 44 16809785
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 44 23345524
Urn1 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 43 21386897
B complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 43 24448447
P complex on AdML, short 3' exon, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 41 23345524
Post-catalytic complex, deltaPrp22 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 41 23431055
Prp43 dissasociated ILS 5-6 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 41 23431055
Bact complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 40 19941820
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 40 18981222
B complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 38 21536652
Bact complex, deltaPrp2 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 38 23431055
Prp43 dissasociated ILS 11-12 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 38 23431055
B complex Sample#2, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 38 27377154
Prp43 dissasociated ILS 3-4 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 34 23431055
C complex on ActinACAC, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 33 19941820
Prp43 dissasociated ILS 1-2 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 31 23431055
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 29 18981222
U2/U5/U6 complex CDC5-tag, S. pombe, (Ohi et al 2002) S. pombe 28 11884590
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 27 18322460
delta prp22 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 27 20089683
Prp43 dissasociated ILS 15-16 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 27 23431055
Clf1p-pulldown, S. cerevisiae, (Wang et al 2003) S. cerevisiae 26 12509417
C complex, H. sapiens, (Jurica et al 2002) H. sapiens 25 11991638
Prp43 dissasociated ILS 13-14 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 25 23431055
U2/U5/U6 complex Cwc1-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 19 11884590
U2/U5/U6 complex Prp19-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 18 11884590
Bact complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 18 19941820
Pre-B complex Sample#3, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 18 27377154
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 17 18981222
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 17 18981222
CACN-1 pulldown with FLAG-HA tandem affinity from C. elegans, C. elegans, (Doherty et al 2014) C. elegans 17 24948787
U2/U5/U6 complex CDC5-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 16 11884590
B complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 16 19941820
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 16 18981222
B complex via MS2 tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 16 16809785
Supraspliceosome, G. gallus, (Chen et al 2007) G. gallus 15 17537823
delta prp16 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 15 20089683
delta prp2 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 14 20089683
B complex on Ftz, sample 1, H. sapiens, (Herold et al 2009) H. sapiens 14 18981222
B complex via tobramycin tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 14 16809785
Dre4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 14 21386897
B complex on Ftz, sample 2, H. sapiens, (Herold et al 2009) H. sapiens 12 18981222
penta-snRNP, S. cerevisiae, (Stevens et al 2002) S. cerevisiae 12 11804584
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 12 12226669
U2/U5/U6 complex Snt309-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 9 11884590
Pre-B complex Sample#2, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 9 27377154
Released with ILS, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 8 23431055
Pre-B complex Sample#1, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 8 27377154
U2/U5/U6 complex Cwc2-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 7 11884590
B complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 6 19941820
C complex on AdML, 3M urea wash, H. sapiens, (Coltri et al 2011) H. sapiens 6 21526149
C complex on AdML, maltose elute, H. sapiens, (Coltri et al 2011) H. sapiens 6 21526149
B complex via tobramycin tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 5 16809785
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 4 12226669
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 4 17537823
Aim4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 4 21386897
B complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 1 18981222
U5 35S complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
B* complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
Mixed spliceosome complexes, H. sapiens, (Neubauer et al 1999) H. sapiens ■ 9731529
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931

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