Nomenclature

Gene Symbol
SF3A1 (H. sapiens)
Other Gene Symbols
PRP21, PRPF21, SAP114, SF3A120
Full Name
splicing factor 3a, subunit 1, 120kDa
Other Names
SF3a120, SAP 114, pre-mRNA processing 21, pre-mRNA splicing factor SF3a (120 kDa subunit), spliceosome associated protein 114, spliceosome-associated protein 114

Other Resources

Entrez: Gene Info
10291
Uniprot: Protein Info
SF3A1_HUMAN
Organism-Specific Database
—
BioGrid: Interaction Info
115580
PDB: Structures
1ZKH, 2DT6, 2DT7
SpliProt3D: Structural Model
SF3A1

Gene Product Info

Molecular Weight (kD)
88.9
Domains
Ubiquitin-like
Motifs
—
Features
—
Complex Association
17S U2 snRNP, A complex, B complex, Bact complex, C complex, P complex
Class / Family
17S U2 snRNP
Notes
—

Orthologs

Name Organism
PRP21 S. cerevisiae
sap114 S. pombe
CG16941 D. melanogaster
W07E6.4 C. elegans
SF3A1 G. gallus

View orthologs at Homologene

Interactions
(from Hegele et al, 2012)

Interacting Partner 1 Interacting Partner 2 Interaction Type Score PubMed ID
SF3A1 DDX42 Y2H: Bait/Prey 0.67 22365833
SF3A1 SF3A2 Dervied from Literature — 11533230
SF3A1 SF3A2 Y2H: Prey/Bait 0.33 22365833
SF3A1 SF3A2 Pull-down — 22365833
SF3A1 SF3A3 Dervied from Literature — 8022796, 11533230, 17098193
SF3A1 SF3A3 Pull-down — 22365833
SF3A1 SF3A3 Y2H: Bidirectional 1 22365833
SF3A1 SF1 Y2H: Bait/Prey 0.78 22365833
SF3A1 PRPF40A Pull-down — 22365833
SF3A1 PRPF40A Y2H: Prey/Bait 0.5 22365833
SF3A1 RBFOX2 Y2H: Bait/Prey 0.67 22365833
SF3A1 RBM10 Y2H: Bait/Prey 1 22365833
SF3A1 RBM10 Pull-down — 22365833

view interactions at SSPPIR...

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
B complex Sample#3, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 213 27377154
17S U2 snRNP, H. sapiens, (Agafonov et al 2011) H. sapiens 186 21536652
Pre-B complex Sample#3, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 185 27377154
A complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 175 21536652
B complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 159 21536652
Bact complex on PM5-20, H. sapiens, (Agafonov et al 2011) H. sapiens 128 21536652
Bact complex on PM5-20, H. sapiens, (Bessonov et al 2010) H. sapiens 125 20980672
Bact complex on PM5-10, H. sapiens, (Bessonov et al 2010) H. sapiens 111 20980672
B complex Sample#2, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 88 27377154
B complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 84 18322460
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 84 18981222
Bact complex, deltaPrp2 on M3Actin, Orbitrap, 0.25 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 78 19935684
ILS, released intron complex on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 77 23431055
Pre-B complex Sample#1, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 75 27377154
B complex via MS2 tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 71 16809785
B complex via tobramycin tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 71 16809785
Pre-B complex Sample#2, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 70 27377154
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 66 19935684
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 63 19935684
B complex Sample#1, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 63 27377154
B complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 59 24448447
B complex via tobramycin tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 58 16809785
A complex, H. sapiens, (Behzadnia et al 2007) H. sapiens 53 17332742
Prp19 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 53 21386897
B complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 50 19941820
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 50 19935684
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 47 18322460
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 46 19935684
45S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 45 19935684
B complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 45 24448447
C complex on PM5, H. sapiens, (Agafonov et al 2011) H. sapiens 44 21536652
B complex via MS2 tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 43 16809785
C complex on PM5, H. sapiens, (Bessonov et al 2010) H. sapiens 40 20980672
Dre4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 40 21386897
U2 snRNP IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 40 22110043
C complex on MINX-GG, H. sapiens, (Bessonov et al 2010) H. sapiens 39 20980672
E complex IP'd by antibody to U1A, H. sapiens, (Marakov et al 2011) H. sapiens 38 22110043
Saf3 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 36 21386897
Bact complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 35 19941820
B complex on Ftz, sample 2, H. sapiens, (Herold et al 2009) H. sapiens 32 18981222
45SBact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 32 19935684
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 28 18981222
Post-catalytic complex, deltaPrp22 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 28 23431055
Prp43 dissasociated ILS 5-6 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 28 23431055
Released with ILS, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 27 23431055
Prp43 dissasociated ILS 3-4 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 27 23431055
B complex on Ftz, sample 1, H. sapiens, (Herold et al 2009) H. sapiens 26 18981222
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 26 18981222
Bact complex, deltaPrp2 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 25 23431055
E-like complex (E') Weri, H. sapiens, (Sharma et al 2008) H. sapiens 24 18193060
Bact complex, deltaPrp2 on M3Actin, Q-Tof, 1 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 24 19935684
Prp43 dissasociated ILS 11-12 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 22 23431055
Supraspliceosome, G. gallus, (Chen et al 2007) G. gallus 21 17537823
B complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 21 18981222
Prp43 dissasociated ILS 9-10 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 21 23431055
E-like complex (E') HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 20 18193060
C complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 20 18981222
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 20 18981222
EDC-A complex Weri, H. sapiens, (Sharma et al 2008) H. sapiens 19 18193060
E complex IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 19 22110043
Prp43 dissasociated ILS 1-2 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 18 23431055
EDC-A complex HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 17 18193060
Prp43 dissasociated ILS 13-14 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 17 23431055
C complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 17 24448447
Mixed complexes Weri, H. sapiens, (Sharma et al 2008) H. sapiens 16 18193060
Bact complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 16 19941820
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 16 12226669
P complex on AdML, short 3' exon, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 16 23345524
C complex on ActinACAC, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 14 19941820
delta prp2 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 13 20089683
penta-snRNP, S. cerevisiae, (Stevens et al 2002) S. cerevisiae 13 11804584
C complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 13 18322460
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 13 12226669
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 12 23345524
C complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 11 18322460
A-like complex (A') HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 11 18193060
C complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 11 24448447
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 10 17537823
EDC-E complex Weri, H. sapiens, (Sharma et al 2008) H. sapiens 10 18193060
Prp43 dissasociated ILS 15-16 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 10 23431055
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 9 18981222
C complex exons trimmed, H. sapiens, (Ilagan et al 2009) H. sapiens 8 19761775
C complex, H. sapiens, (Jurica et al 2002) H. sapiens 8 11991638
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 8 19935684
EDC-E complex HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 7 18193060
C complex, H. sapiens, (Ilagan et al 2009) H. sapiens 6 19761775
C complex 1M NaCl-washed core, H. sapiens, (Bessonov et al 2008) H. sapiens 6 18322460
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 5 18322460
C complex on ActinD31, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 5 19941820
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 5 19935684
B complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 4 19941820
C complex on AdML, maltose elute, H. sapiens, (Coltri et al 2011) H. sapiens 4 21526149
Prp43 dissasociated ILS 7-8 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 4 23431055
H complex, H. sapiens, (Jurica et al 2002) H. sapiens 3 11991638
U2/U5/U6 complex CDC5-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 2 11884590
U2/U5/U6 complex Prp19-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 2 11884590
mRNP on BetaGlobin, H. sapiens, (Merz et al 2007) H. sapiens 2 17095540
mRNP on AdML, H. sapiens, (Merz et al 2007) H. sapiens 2 17095540
Cwc21 tap-tag pulldown, S. cerevisiae, (Khanna et al 2009) S. cerevisiae 2 19789211
Prp8 tap-tag pulldown, S. cerevisiae, (Khanna et al 2009) S. cerevisiae 2 19789211
C complex on AdML, 3M urea wash, H. sapiens, (Coltri et al 2011) H. sapiens 2 21526149
U2/U5/U6 complex CDC5-tag, S. pombe, (Ohi et al 2002) S. pombe 1 11884590
U2 17S, H. sapiens, (Will et al 2002) H. sapiens ■ 12234937
E complex Weri, H. sapiens, (Sharma et al 2005) H. sapiens ■ 16109373
B* complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931
CDC5L pulldown, H. sapiens, (Ajuh et al 1999) H. sapiens ■ 11101529
A complex, H. sapiens, (Hartmuth et al 2002) H. sapiens ■ 12477934
Prp1 tap-tag pulldown, S. pombe, (Carnahan et al 2005) S. pombe ■ 15755920
PSF-pulldown, without splicing substrate, H. sapiens, (Peng et al 2006) H. sapiens ■ 17114945
PSF-pulldown, with AdMl splicing subtrate, H. sapiens, (Peng et al 2006) H. sapiens ■ 17114945
B complex on MINX-MS2, ATPgammaS block, 40S glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562
A complex on MINX-MS2, ATPgammaS block, glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562

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