Nomenclature

Gene Symbol
SF3A2 (H. sapiens)
Other Gene Symbols
PRP11, PRPF11, SAP62, SF3a66
Full Name
splicing factor 3a, subunit 2, 66kDa
Other Names
SF3a66, SAP 62, pre-mRNA splicing factor SF3A, subunit 2, spliceosome associated protein 62, spliceosome-associated protein 62, splicing factor 3A subunit 2

Other Resources

Entrez: Gene Info
8175
Uniprot: Protein Info
SF3A2_HUMAN
Organism-Specific Database
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BioGrid: Interaction Info
113826
PDB: Structures
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SpliProt3D: Structural Model
SF3A2

Gene Product Info

Molecular Weight (kD)
49.3
Domains
—
Motifs
—
Features
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Complex Association
17S U2 snRNP, A complex, B complex, Bact complex, C complex, P complex
Class / Family
17S U2 snRNP
Notes
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Orthologs

Name Organism
PRP11 S. cerevisiae
sap62 S. pombe
CG10754 D. melanogaster
F11A10.2 C. elegans
SF3A2 G. gallus

View orthologs at Homologene

Interactions
(from Hegele et al, 2012)

Interacting Partner 1 Interacting Partner 2 Interaction Type Score PubMed ID
SF3A2 SF3A1 Y2H: Bait/Prey 0.33 22365833
SF3A2 SF3A1 Pull-down — 22365833
SF3A2 SF3A1 Dervied from Literature — 11533230
SF3A2 HTATSF1 Dervied from Literature — 9710584

view interactions at SSPPIR...

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
B complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 134 21536652
Bact complex on PM5-20, H. sapiens, (Agafonov et al 2011) H. sapiens 125 21536652
17S U2 snRNP, H. sapiens, (Agafonov et al 2011) H. sapiens 117 21536652
A complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 100 21536652
B complex via MS2 tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 56 16809785
ILS, released intron complex on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 48 23431055
Bact complex, deltaPrp2 on M3Actin, Orbitrap, 0.25 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 47 19935684
B complex Sample#3, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 40 27377154
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 34 19935684
C complex on PM5, H. sapiens, (Agafonov et al 2011) H. sapiens 33 21536652
Pre-B complex Sample#3, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 32 27377154
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 31 19935684
B complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 30 19941820
Bact complex on PM5-10, H. sapiens, (Bessonov et al 2010) H. sapiens 30 20980672
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 30 19935684
Dre4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 29 21386897
45SBact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 29 19935684
Prp19 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 27 21386897
Saf3 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 27 21386897
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 27 19935684
Bact complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 25 19941820
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 25 18322460
B complex via MS2 tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 24 16809785
45S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 24 19935684
Prp43 dissasociated ILS 9-10 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 23 23431055
B complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 23 24448447
Bact complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 22 19941820
Post-catalytic complex, deltaPrp22 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 21 23431055
Bact complex, deltaPrp2 on M3Actin, Q-Tof, 1 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 20 19935684
B complex Sample#2, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 20 27377154
B complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 18 18322460
Bact complex, deltaPrp2 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 18 23431055
Prp43 dissasociated ILS 7-8 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 18 23431055
Pre-B complex Sample#2, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 17 27377154
Prp43 dissasociated ILS 5-6 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 15 23431055
Prp43 dissasociated ILS 11-12 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 15 23431055
Pre-B complex Sample#1, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 15 27377154
Prp43 dissasociated ILS 1-2 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 14 23431055
A complex, H. sapiens, (Behzadnia et al 2007) H. sapiens 13 17332742
B complex via tobramycin tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 13 16809785
B complex via tobramycin tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 13 16809785
B complex Sample#1, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 13 27377154
Prp43 dissasociated ILS 3-4 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 12 23431055
penta-snRNP, S. cerevisiae, (Stevens et al 2002) S. cerevisiae 11 11804584
Prp43 dissasociated ILS 13-14 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 11 23431055
Bact complex on PM5-20, H. sapiens, (Bessonov et al 2010) H. sapiens 10 20980672
E complex IP'd by antibody to U1A, H. sapiens, (Marakov et al 2011) H. sapiens 10 22110043
delta prp2 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 9 20089683
C complex on ActinACAC, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 9 19941820
C complex on MINX-GG, H. sapiens, (Bessonov et al 2010) H. sapiens 9 20980672
A-like complex (A') HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 9 18193060
B complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 9 24448447
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 8 19935684
P complex on AdML, short 3' exon, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 8 23345524
E complex IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 8 22110043
U2 snRNP IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 8 22110043
Released with ILS, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 8 23431055
Prp43 dissasociated ILS 15-16 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 8 23431055
Mixed complexes Weri, H. sapiens, (Sharma et al 2008) H. sapiens 7 18193060
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 6 18981222
B complex on Ftz, sample 2, H. sapiens, (Herold et al 2009) H. sapiens 5 18981222
B complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 5 19941820
Supraspliceosome, G. gallus, (Chen et al 2007) G. gallus 5 17537823
E-like complex (E') HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 5 18193060
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 5 18981222
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 4 18322460
C complex on PM5, H. sapiens, (Bessonov et al 2010) H. sapiens 4 20980672
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 4 12226669
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 4 17537823
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 4 18981222
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 4 18981222
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 4 19935684
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 4 23345524
U2/U5/U6 complex Prp19-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 3 11884590
C complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 3 18322460
U2/U5/U6 complex CDC5-tag, S. pombe, (Ohi et al 2002) S. pombe 3 11884590
E-like complex (E') Weri, H. sapiens, (Sharma et al 2008) H. sapiens 3 18193060
EDC-A complex Weri, H. sapiens, (Sharma et al 2008) H. sapiens 3 18193060
B complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 3 18981222
C complex on ActinD31, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 2 19941820
EDC-E complex HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 2 18193060
C complex, H. sapiens, (Jurica et al 2002) H. sapiens 2 11991638
EDC-E complex Weri, H. sapiens, (Sharma et al 2008) H. sapiens 2 18193060
B complex on Ftz, sample 1, H. sapiens, (Herold et al 2009) H. sapiens 2 18981222
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 2 18981222
C complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 2 24448447
C complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 2 24448447
U2/U5/U6 complex CDC5-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 1 11884590
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 1 12226669
U2 17S, H. sapiens, (Will et al 2002) H. sapiens ■ 12234937
E complex Weri, H. sapiens, (Sharma et al 2005) H. sapiens ■ 16109373
B* complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
Mixed spliceosome complexes, H. sapiens, (Neubauer et al 1999) H. sapiens ■ 9731529
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931
A complex, H. sapiens, (Hartmuth et al 2002) H. sapiens ■ 12477934
Prp1 tap-tag pulldown, S. pombe, (Carnahan et al 2005) S. pombe ■ 15755920
PSF-pulldown, with AdMl splicing subtrate, H. sapiens, (Peng et al 2006) H. sapiens ■ 17114945
B complex on MINX-MS2, ATPgammaS block, 40S glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562
A complex on MINX-MS2, ATPgammaS block, glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562

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