Nomenclature

Gene Symbol
PRP8 (S. cerevisiae)
Other Gene Symbols
DBF3, DNA39, RNA8, SLT21, USA2
Full Name
Prp8p
Other Names
PRP8, YHR165C

Other Resources

Entrez: Gene Info
856570
Uniprot: Protein Info
PRP8_YEAST
Organism-Specific Database
S000001208
BioGrid: Interaction Info
36599
PDB: Structures
2OG4, 3E66, 3E9O, 3E9P, 3SBT, 4I43
SpliProt3D: Structural Model
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Gene Product Info

Molecular Weight (kD)
279.5
Domains
MPN
Motifs
—
Features
—
Complex Association
B complex, Bact complex, C complex, P complex, tri-snRNP, U5 snRNP
Class / Family
U5 snRNP
Notes
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Orthologs

Name Organism
PRPF8 H. sapiens
spp42 S. pombe
Prp8 D. melanogaster
C50C3.6 C. elegans
PFD0265w P. falciparum
PRPF8 G. gallus

View orthologs at Homologene

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
Prp19 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 2542 21386897
Cwf2 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 2392 21386897
Prp17tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 2179 21386897
B complex Sample#3, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 1601 27377154
Pre-B complex Sample#3, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 1048 27377154
Bact complex on PM5-20, H. sapiens, (Bessonov et al 2010) H. sapiens 704 20980672
C complex on MINX-GG, H. sapiens, (Bessonov et al 2010) H. sapiens 556 20980672
Bact complex, deltaPrp2 on M3Actin, Orbitrap, 0.25 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 533 19935684
Bact complex on PM5-10, H. sapiens, (Bessonov et al 2010) H. sapiens 523 20980672
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 480 19935684
C complex on PM5, H. sapiens, (Bessonov et al 2010) H. sapiens 451 20980672
B complex Sample#2, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 450 27377154
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 434 19935684
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 430 19935684
B complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 408 18322460
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 404 19935684
B complex Sample#1, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 392 27377154
45S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 386 19935684
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 378 19935684
45SBact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 378 19935684
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 374 19935684
B complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 345 24448447
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 335 18322460
ILS, released intron complex on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 322 23431055
B complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 316 24448447
C complex on ActinACAC, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 306 19941820
B complex via MS2 tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 306 16809785
C complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 299 18322460
Pre-B complex Sample#2, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 271 27377154
C complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 261 18322460
Pre-B complex Sample#1, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 257 27377154
Prp43 dissasociated ILS 7-8 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 242 23431055
C complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 240 24448447
Bact complex, deltaPrp2 on M3Actin, Q-Tof, 1 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 236 19935684
B complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 233 19941820
Prp43 dissasociated ILS 11-12 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 209 23431055
C complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 202 18981222
C complex 1M NaCl-washed core, H. sapiens, (Bessonov et al 2008) H. sapiens 193 18322460
B complex via MS2 tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 184 16809785
C complex on ActinD31, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 176 19941820
Prp43 dissasociated ILS 5-6 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 176 23431055
Post-catalytic complex, deltaPrp22 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 175 23431055
Bact complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 166 19941820
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 161 18981222
Prp43 dissasociated ILS 9-10 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 156 23431055
C complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 153 24448447
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 150 18981222
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 149 18981222
Dre4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 146 21386897
B complex on Ftz, sample 2, H. sapiens, (Herold et al 2009) H. sapiens 140 18981222
Bact complex, deltaPrp2 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 137 23431055
P complex on AdML, short 3' exon, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 127 23345524
Saf3 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 126 21386897
Prp43 dissasociated ILS 13-14 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 126 23431055
Bact complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 125 19941820
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 123 18981222
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 123 23345524
Prp43 dissasociated ILS 3-4 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 122 23431055
B complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 111 21536652
Prp43 dissasociated ILS 15-16 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 109 23431055
C complex on PM5, H. sapiens, (Agafonov et al 2011) H. sapiens 107 21536652
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 104 18981222
tri-snRNP, H. sapiens, (Agafonov et al 2011) H. sapiens 104 21536652
Bact complex on PM5-20, H. sapiens, (Agafonov et al 2011) H. sapiens 95 21536652
B complex on Ftz, sample 1, H. sapiens, (Herold et al 2009) H. sapiens 92 18981222
U2/U5/U6 complex CDC5-tag, S. pombe, (Ohi et al 2002) S. pombe 92 11884590
B complex via tobramycin tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 91 16809785
C complex, H. sapiens, (Ilagan et al 2009) H. sapiens 88 19761775
B complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 81 19941820
C complex on AdML, 3M urea wash, H. sapiens, (Coltri et al 2011) H. sapiens 77 21526149
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 72 17537823
Supraspliceosome, G. gallus, (Chen et al 2007) G. gallus 72 17537823
B complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 71 18981222
U2/U5/U6 complex Prp19-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 67 11884590
Prp8 tap-tag pulldown, S. cerevisiae, (Khanna et al 2009) S. cerevisiae 62 19789211
delta prp2 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 60 20089683
Urn1 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 52 21386897
E complex IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 52 22110043
Prp43 dissasociated ILS 1-2 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 48 23431055
delta prp22 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 44 20089683
SKIP GST-tag pulldown, H. sapiens, (Zhang et al 2003) H. sapiens 44 12840015
delta prp16 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 43 20089683
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 42 18322460
C complex, H. sapiens, (Jurica et al 2002) H. sapiens 41 11991638
U2/U5/U6 complex CDC5-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 38 11884590
A complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 38 21536652
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 33 12226669
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 29 12226669
C complex exons trimmed, H. sapiens, (Ilagan et al 2009) H. sapiens 28 19761775
CACN-1 pulldown with FLAG-HA tandem affinity from C. elegans, C. elegans, (Doherty et al 2014) C. elegans 28 24948787
C complex on AdML, maltose elute, H. sapiens, (Coltri et al 2011) H. sapiens 25 21526149
Saf2 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 24 21386897
penta-snRNP, S. cerevisiae, (Stevens et al 2002) S. cerevisiae 22 11804584
Clf1p-pulldown, S. cerevisiae, (Wang et al 2003) S. cerevisiae 21 12509417
U2/U5/U6 complex Snt309-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 18 11884590
Mixed complexes Weri, H. sapiens, (Sharma et al 2008) H. sapiens 17 18193060
U2/U5/U6 complex Cwc1-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 16 11884590
H complex, H. sapiens, (Merz et al 2007) H. sapiens 12 17095540
Saf1 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 10 21386897
mRNP on AdML, H. sapiens, (Merz et al 2007) H. sapiens 8 17095540
U2/U5/U6 complex Cwc2-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 7 11884590
Cwc21 tap-tag pulldown, S. cerevisiae, (Khanna et al 2009) S. cerevisiae 3 19789211
SRM160 pulldown with BIC8 antibody, H. sapiens, (McCracken et al 2005) H. sapiens 3 16159877
mRNP on short 5' exon, AdML, H. sapiens, (Merz et al 2007) H. sapiens 2 17095540
mRNP unspliced control, H. sapiens, (Merz et al 2007) H. sapiens 2 17095540
B complex via tobramycin tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 2 16809785
U2 snRNP IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 2 22110043
Released from Post-catalytic complex, deltaPrp22, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 2 23431055
mRNP on BetaGlobin, H. sapiens, (Merz et al 2007) H. sapiens 1 17095540
H complex, H. sapiens, (Jurica et al 2002) H. sapiens 1 11991638
Released with ILS, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 1 23431055
tri-snRNP, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
tri-snRNP, S. cerevisiae, (Stevens et al 1999) S. cerevisiae ■ 11720284
U5 35S complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
tri-snRNP, S. cerevisiae, (Gottschalk et al 1999) S. cerevisiae ■ 10449419
B* complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931
A complex, H. sapiens, (Hartmuth et al 2002) H. sapiens ■ 12477934
Prp1 tap-tag pulldown, S. pombe, (Carnahan et al 2005) S. pombe ■ 15755920
PSF-pulldown, without splicing substrate, H. sapiens, (Peng et al 2006) H. sapiens ■ 17114945
B complex on MINX-MS2, ATPgammaS block, 40S glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562

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