Nomenclature

Gene Symbol
BCAS2 (H. sapiens)
Other Gene Symbols
DAM1, SPF27, Snt309
Full Name
breast carcinoma amplified sequence 2
Other Names
BCAS2, DNA amplified in mammary carcinoma 1 protein, breast carcinoma-amplified sequence 2, pre-mRNA-splicing factor SPF27, spliceosome associated protein, amplified in breast cancer, spliceosome-associated protein SPF 27

Other Resources

Entrez: Gene Info
10286
Uniprot: Protein Info
SPF27_HUMAN
Organism-Specific Database
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BioGrid: Interaction Info
115575
PDB: Structures
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SpliProt3D: Structural Model
BCAS2

Gene Product Info

Molecular Weight (kD)
26.1
Domains
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Motifs
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Features
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Complex Association
B complex, Bact complex, C complex, P complex
Class / Family
PRP19 complex
Notes
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Orthologs

Name Organism
SNT309 S. cerevisiae
cwf7 S. pombe
CG4980 D. melanogaster
T12A2.7 C. elegans

View orthologs at Homologene

Interactions
(from Hegele et al, 2012)

Interacting Partner 1 Interacting Partner 2 Interaction Type Score PubMed ID
BCAS2 SF3B4 Pull-down — 22365833
BCAS2 SF3B4 Y2H: Prey/Bait 1 22365833
BCAS2 SF1 Y2H: Bidirectional 1 22365833
BCAS2 PRPF8 Pull-down — 22365833
BCAS2 PRPF8 Y2H: Prey/Bait 0.67 22365833
BCAS2 PRPF19 Pull-down — 22365833
BCAS2 PRPF19 Dervied from Literature — 20176811
BCAS2 PRPF19 Y2H: Prey/Bait 0.75 22365833
BCAS2 CDC5L Dervied from Literature — 20176811
BCAS2 CDC5L Y2H: Bidirectional 0.62 22365833

view interactions at SSPPIR...

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
Cwf2 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 353 21386897
Prp19 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 336 21386897
Prp17tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 249 21386897
C complex on PM5, H. sapiens, (Agafonov et al 2011) H. sapiens 242 21536652
Bact complex on PM5-20, H. sapiens, (Agafonov et al 2011) H. sapiens 230 21536652
B complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 63 21536652
C complex on MINX-GG, H. sapiens, (Bessonov et al 2010) H. sapiens 50 20980672
Bact complex on PM5-20, H. sapiens, (Bessonov et al 2010) H. sapiens 41 20980672
Bact complex on PM5-10, H. sapiens, (Bessonov et al 2010) H. sapiens 39 20980672
Urn1 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 39 21386897
B complex Sample#3, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 37 27377154
C complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 34 18322460
C complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 33 24448447
B complex Sample#2, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 31 27377154
C complex 1M NaCl-washed core, H. sapiens, (Bessonov et al 2008) H. sapiens 27 18322460
C complex on PM5, H. sapiens, (Bessonov et al 2010) H. sapiens 27 20980672
Bact complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 21 19941820
B complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 21 18322460
B complex via MS2 tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 21 16809785
B complex via MS2 tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 20 16809785
45S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 20 19935684
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 20 19935684
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 20 19935684
B complex Sample#1, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 20 27377154
P complex on AdML, short 3' exon, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 19 23345524
B complex via tobramycin tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 18 16809785
C complex on ActinD31, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 17 19941820
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 17 18981222
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 17 23345524
Bact complex, deltaPrp2 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 17 23431055
B complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 17 24448447
U2/U5/U6 complex CDC5-tag, S. pombe, (Ohi et al 2002) S. pombe 15 11884590
ILS, released intron complex on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 15 23431055
Post-catalytic complex, deltaPrp22 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 14 23431055
Prp43 dissasociated ILS 3-4 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 14 23431055
delta prp2 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 13 20089683
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 13 19935684
Bact complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 11 19941820
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 11 18322460
C complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 11 18981222
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 11 18981222
Aim4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 11 21386897
B complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 11 24448447
C complex, H. sapiens, (Ilagan et al 2009) H. sapiens 10 19761775
C complex on ActinACAC, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 10 19941820
delta prp22 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 10 20089683
C complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 9 18322460
C complex on AdML, 3M urea wash, H. sapiens, (Coltri et al 2011) H. sapiens 9 21526149
C complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 9 24448447
Clf1p-pulldown, S. cerevisiae, (Wang et al 2003) S. cerevisiae 8 12509417
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 7 18981222
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 6 12226669
B complex on Ftz, sample 1, H. sapiens, (Herold et al 2009) H. sapiens 6 18981222
B complex via tobramycin tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 6 16809785
Bact complex, deltaPrp2 on M3Actin, Orbitrap, 0.25 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 6 19935684
Prp43 dissasociated ILS 5-6 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 6 23431055
Pre-B complex Sample#3, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 6 27377154
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 5 18322460
delta prp16 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 5 20089683
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 5 12226669
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 5 18981222
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 5 18981222
B complex on Ftz, sample 2, H. sapiens, (Herold et al 2009) H. sapiens 4 18981222
Prp43 dissasociated ILS 1-2 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 4 23431055
Pre-B complex Sample#2, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 4 27377154
Mixed complexes Weri, H. sapiens, (Sharma et al 2008) H. sapiens 3 18193060
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 3 17537823
B complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 2 19941820
B complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 2 18981222
Dre4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 2 21386897
Prp43 dissasociated ILS 7-8 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 2 23431055
Prp43 dissasociated ILS 13-14 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 2 23431055
Pre-B complex Sample#1, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 2 27377154
C complex exons trimmed, H. sapiens, (Ilagan et al 2009) H. sapiens 1 19761775
B complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 1 19941820
Cwc21 tap-tag pulldown, S. cerevisiae, (Khanna et al 2009) S. cerevisiae 1 19789211
Prp8 tap-tag pulldown, S. cerevisiae, (Khanna et al 2009) S. cerevisiae 1 19789211
45SBact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 1 19935684
E complex IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 1 22110043
C complex on AdML, maltose elute, H. sapiens, (Coltri et al 2011) H. sapiens 1 21526149
Prp43 dissasociated ILS 9-10 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 1 23431055
Prp43 dissasociated ILS 11-12 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 1 23431055
Prp43 dissasociated ILS 15-16 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 1 23431055
U5 35S complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
B* complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
Mixed spliceosome complexes, H. sapiens, (Neubauer et al 1999) H. sapiens ■ 9731529
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931
CDC5L pulldown, H. sapiens, (Ajuh et al 1999) H. sapiens ■ 11101529
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae ■ 19935684
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae ■ 19935684
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae ■ 19935684

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