Nomenclature

Gene Symbol
PUF60 (H. sapiens)
Other Gene Symbols
FIR, FLJ31379, RoBPI, SIAHBP1
Full Name
poly-U binding splicing factor 60KDa
Other Names
PUF60, 60 kDa poly(U)-binding-splicing factor, FBP interacting repressor, FBP-interacting repressor, Ro ribonucleoprotein binding protein 1, Ro ribonucleoprotein-binding protein 1

Other Resources

Entrez: Gene Info
22827
Uniprot: Protein Info
PUF60_HUMAN
Organism-Specific Database
—
BioGrid: Interaction Info
116502
PDB: Structures
2DNY, 2KXF, 2KXH, 2QFJ, 3DXB
SpliProt3D: Structural Model
PUF60

Gene Product Info

Molecular Weight (kD)
59.9
Domains
RRM, RRM 3; atypical
Motifs
—
Features
—
Complex Association
A complex
Class / Family
17S U2 snRNP associated
Notes
—

Orthologs

Name Organism
pUf68 D. melanogaster
Y47G6A.20 C. elegans
PUF60 G. gallus

View orthologs at Homologene

Interactions
(from Hegele et al, 2012)

Interacting Partner 1 Interacting Partner 2 Interaction Type Score PubMed ID
PUF60 SNRPF Y2H: Prey/Bait 0.72 22365833
PUF60 U2AF2 Dervied from Literature — 10866799, 16169070, 16189514
PUF60 U2AF2 Y2H: Prey/Bait 0.62 22365833
PUF60 PUF60 Y2H: Homodimer 0.5 22365833
PUF60 PUF60 Dervied from Literature — 16189514
PUF60 PTBP2 Y2H: Prey/Bait 0.75 22365833
PUF60 PCBP1 Dervied from Literature — 16713569
PUF60 SAP30BP Dervied from Literature — 16189514, 18255255
PUF60 SAP30BP Y2H: Bidirectional 1 22365833

view interactions at SSPPIR...

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
A complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 126 21536652
C complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 29 18981222
E complex IP'd by antibody to U1A, H. sapiens, (Marakov et al 2011) H. sapiens 29 22110043
E-like complex (E') HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 27 18193060
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 25 18981222
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 20 18981222
E-like complex (E') Weri, H. sapiens, (Sharma et al 2008) H. sapiens 19 18193060
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 17 18981222
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 16 18322460
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 16 18981222
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 15 12226669
E complex IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 13 22110043
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 12 18981222
Mixed complexes Weri, H. sapiens, (Sharma et al 2008) H. sapiens 11 18193060
A complex, H. sapiens, (Behzadnia et al 2007) H. sapiens 10 17332742
EDC-E complex Weri, H. sapiens, (Sharma et al 2008) H. sapiens 10 18193060
EDC-E complex HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 9 18193060
B complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 9 18981222
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 8 18322460
EDC-A complex Weri, H. sapiens, (Sharma et al 2008) H. sapiens 8 18193060
EDC-A complex HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 8 18193060
Pre-B complex Sample#2, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 8 27377154
A-like complex (A') HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 7 18193060
B complex Sample#2, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 7 27377154
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 5 12226669
Pre-B complex Sample#3, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 4 27377154
B complex on Ftz, sample 2, H. sapiens, (Herold et al 2009) H. sapiens 3 18981222
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 3 17537823
U2 snRNP IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 3 22110043
SRM160 pulldown with BIC8 antibody, H. sapiens, (McCracken et al 2005) H. sapiens 3 16159877
H complex, H. sapiens, (Jurica et al 2002) H. sapiens 1 11991638
C complex, H. sapiens, (Jurica et al 2002) H. sapiens 1 11991638
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 1 23345524
Pre-B complex Sample#1, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 1 27377154
B complex Sample#3, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 1 27377154
H complex, H. sapiens, (Sharma et al 2005) H. sapiens ■ 16109373
U2 17S, H. sapiens, (Will et al 2002) H. sapiens ■ 12234937
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931
A complex on MINX-MS2, ATPgammaS block, glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562

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