Nomenclature

Gene Symbol
DHX8 (H. sapiens)
Other Gene Symbols
DDX8, HRH1, PRP22, PRPF22
Full Name
DEAH (Asp-Glu-Ala-His) box polypeptide 8
Other Names
PRP22, ATP-dependent RNA helicase DHX8, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase), DEAH box protein 8, DEAH-box protein 8, RNA helicase HRH1

Other Resources

Entrez: Gene Info
1659
Uniprot: Protein Info
DHX8_HUMAN
Organism-Specific Database
—
BioGrid: Interaction Info
108024
PDB: Structures
2EQS, 3I4U
SpliProt3D: Structural Model
DHX8

Gene Product Info

Molecular Weight (kD)
139.3
Domains
Helicase ATP-binding, Helicase C-terminal, S1 motif
Motifs
DEAH box
Features
—
Complex Association
C complex, P complex
Class / Family
Second step factors
Notes
—

Orthologs

Name Organism
PRP22 S. cerevisiae
prp22 S. pombe
pea D. melanogaster
EEED8.5 C. elegans
DHX8 G. gallus

View orthologs at Homologene

Interactions
(from Hegele et al, 2012)

Interacting Partner 1 Interacting Partner 2 Interaction Type Score PubMed ID
DHX8 CHERP Pull-down — 22365833
DHX8 CHERP Y2H: Prey/Bait 1 22365833
DHX8 PRPF40A Y2H: Bidirectional 1 22365833
DHX8 ISY1 Y2H: Bait/Prey 0.67 22365833
DHX8 ISY1 Pull-down — 22365833
DHX8 MFAP1 Y2H: Prey/Bait 0.75 22365833
DHX8 PRPF38A Y2H: Prey/Bait 0.5 22365833
DHX8 DHX8 Y2H: Homodimer 1 22365833
DHX8 DHX8 Pull-down — 22365833
DHX8 DHX8 Pull-down — 22365833
DHX8 NKAP Pull-down — 22365833
DHX8 NKAP Y2H: Bidirectional 0.92 22365833
DHX8 THOC1 Y2H: Prey/Bait 0.5 22365833
DHX8 RBM7 Y2H: Prey/Bait 0.5 22365833
DHX8 RBMX2 Y2H: Prey/Bait 0.67 22365833
DHX8 ZCCHC10 Y2H: Prey/Bait 1 22365833
DHX8 SAP30BP Y2H: Prey/Bait 1 22365833

view interactions at SSPPIR...

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
Prp19 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 234 21386897
C complex on MINX-GG, H. sapiens, (Bessonov et al 2010) H. sapiens 224 20980672
C complex on PM5, H. sapiens, (Bessonov et al 2010) H. sapiens 211 20980672
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 201 18322460
C complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 169 18322460
C complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 160 24448447
C complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 129 18322460
Prp17tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 109 21386897
C complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 97 24448447
delta prp22 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 69 20089683
C complex on ActinACAC, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 68 19941820
Cwf2 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 65 21386897
C complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 54 18981222
C complex on PM5, H. sapiens, (Agafonov et al 2011) H. sapiens 51 21536652
P complex on AdML, short 3' exon, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 44 23345524
C complex, H. sapiens, (Ilagan et al 2009) H. sapiens 40 19761775
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 35 23345524
C complex, H. sapiens, (Jurica et al 2002) H. sapiens 29 11991638
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 28 18981222
C complex on ActinD31, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 24 19941820
U2/U5/U6 complex CDC5-tag, S. pombe, (Ohi et al 2002) S. pombe 16 11884590
Bact complex on PM5-20, H. sapiens, (Bessonov et al 2010) H. sapiens 15 20980672
Clf1p-pulldown, S. cerevisiae, (Wang et al 2003) S. cerevisiae 13 12509417
C complex on AdML, maltose elute, H. sapiens, (Coltri et al 2011) H. sapiens 13 21526149
C complex exons trimmed, H. sapiens, (Ilagan et al 2009) H. sapiens 10 19761775
C complex 1M NaCl-washed core, H. sapiens, (Bessonov et al 2008) H. sapiens 9 18322460
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 9 12226669
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 9 12226669
B complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 9 24448447
U2/U5/U6 complex Prp19-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 8 11884590
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 7 18322460
delta prp16 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 6 20089683
Bact complex on PM5-10, H. sapiens, (Bessonov et al 2010) H. sapiens 6 20980672
C complex on AdML, 3M urea wash, H. sapiens, (Coltri et al 2011) H. sapiens 6 21526149
CACN-1 pulldown with FLAG-HA tandem affinity from C. elegans, C. elegans, (Doherty et al 2014) C. elegans 6 24948787
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 5 18981222
B complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 4 24448447
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 3 18981222
U2/U5/U6 complex CDC5-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 2 11884590
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 2 17537823
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 2 18981222
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 2 18981222
Bact complex, deltaPrp2 on M3Actin, Orbitrap, 0.25 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 1 19935684
Bact complex on PM5-20, H. sapiens, (Agafonov et al 2011) H. sapiens 1 21536652
B* complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931

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