"mass spec experiments" page to find specific MS studies of isolated splicing complexes

An ever-growing number of studies have reported MS/MS analyses of different spliceosome complexes and subunits

Different MS experiments are identified by:

sample type:

the common E, A, B, Bact,C, P complex nomenclature is used when applicable

sess-defined or mixed populations of spliceosomes are designated as "mixed-spliceosomes" or by the component target by which they were purified (i.e. “SMD3 pulldown”)

publication:

first author, last author and year for the experiment published

source organism:

human, yeasts, flies, worms, etc.

Click on individual experiment name to show full results:

protein identified:

aliases, class/family, molecular weight

# of peptides:

the number of sequenced peptides used to identify protein in the experiment serves as a relative measure of confidence/quantification

one caveat of MS data is that they are not strictly quantitative and all proteins reported to associate with a given complex are not necessarily stoichiometric

higher peptide counts correlate with more of the protein in the sample

however, peptide numbers also depend on protein size, with larger proteins yielding more peptides than smaller peptides

the total amount of sample analyzed and the sample complexity, also significantly affect the number of peptides sequenced

it is important to look at the MS data across an entire experiment to get a feel for the number of peptides that indicate likely stoichiometric presence of a protein

for the same reason peptide numbers from different experiments cannot be directly compared

look at data across the entire experiment make judgments about the relative abundance of proteins

Results can be exported to a spreadsheet file