Nomenclature

Gene Symbol
SF3A3 (H. sapiens)
Other Gene Symbols
PRP9, PRPF9, SAP61, SF3a60
Full Name
splicing factor 3a, subunit 3, 60kDa
Other Names
SF3a60, SAP 61, pre-mRNA splicing factor SF3a (60kD), spliceosome associated protein 61, spliceosome-associated protein 61, splicing factor 3A subunit 3

Other Resources

Entrez: Gene Info
10946
Uniprot: Protein Info
SF3A3_HUMAN
Organism-Specific Database
—
BioGrid: Interaction Info
116146
PDB: Structures
2DT7
SpliProt3D: Structural Model
SF3A3

Gene Product Info

Molecular Weight (kD)
58.8
Domains
—
Motifs
—
Features
—
Complex Association
17S U2 snRNP, A complex, B complex, Bact complex, C complex, P complex
Class / Family
17S U2 snRNP
Notes
—

Orthologs

Name Organism
PRP9 S. cerevisiae
sap61 S. pombe
noi D. melanogaster
T13H5.4 C. elegans
SF3A3 G. gallus

View orthologs at Homologene

Interactions
(from Hegele et al, 2012)

Interacting Partner 1 Interacting Partner 2 Interaction Type Score PubMed ID
SF3A3 SF3A1 Pull-down — 22365833
SF3A3 SF3A1 Dervied from Literature — 8022796, 11533230, 17098193
SF3A3 SF3A1 Y2H: Bidirectional 1 22365833
SF3A3 PRMT5 Y2H: Prey/Bait 0.92 22365833

view interactions at SSPPIR...

Identified in the following experiments:

Name Species # Of Peptides PubMed ID
A complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 267 21536652
B complex on MINX, H. sapiens, (Agafonov et al 2011) H. sapiens 240 21536652
17S U2 snRNP, H. sapiens, (Agafonov et al 2011) H. sapiens 221 21536652
Bact complex on PM5-20, H. sapiens, (Agafonov et al 2011) H. sapiens 204 21536652
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 143 19935684
Bact complex, deltaPrp2 on M3Actin, Orbitrap, 0.25 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 137 19935684
45SBact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 136 19935684
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 128 19935684
35S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 123 19935684
ILS, released intron complex on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 120 23431055
B complex Sample#3, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 120 27377154
Prp43 dissasociated ILS 11-12 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 115 23431055
Pre-B complex Sample#3, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 98 27377154
40S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 96 19935684
45S Bact complex, deltaPrp2 (+Prp2/Spp2) on M3Actin, 75 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 94 19935684
Prp43 dissasociated ILS 9-10 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 94 23431055
Prp43 dissasociated ILS 5-6 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 92 23431055
Prp43 dissasociated ILS 7-8 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 90 23431055
Prp43 dissasociated ILS 3-4 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 75 23431055
Bact complex on PM5-10, H. sapiens, (Bessonov et al 2010) H. sapiens 74 20980672
Bact complex on PM5-20, H. sapiens, (Bessonov et al 2010) H. sapiens 72 20980672
C complex on PM5, H. sapiens, (Agafonov et al 2011) H. sapiens 71 21536652
B complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 70 19941820
Prp43 dissasociated ILS 13-14 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 67 23431055
Bact complex sample 1, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 66 19941820
B complex Sample#2, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 63 27377154
B complex via MS2 tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 52 16809785
Post-catalytic complex, deltaPrp22 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 52 23431055
Released with ILS, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 51 23431055
Pre-B complex Sample#1, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 50 27377154
Bact complex, deltaPrp2 on M3Actin, Q-Tof, 1 pmol, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 46 19935684
Prp43 dissasociated ILS 1-2 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 46 23431055
Bact complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 45 19941820
B complex Sample#1, on MINX-MS2 "kinetically" trapped, H. sapiens, (Boesler et al 2015) H. sapiens 45 27377154
Saf3 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 44 21386897
Bact complex, deltaPrp2 on M3Actin7, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 44 23431055
Prp43 dissasociated ILS 15-16 glycerol gradient fraction, S. cerevisiae, (Fourmann et al 2013) S. cerevisiae 44 23431055
B complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 42 18322460
Pre-B complex Sample#2, on MINX-MS2 stalled by DDX23 (hPrp28) depletion, H. sapiens, (Boesler et al 2015) H. sapiens 42 27377154
B complex via tobramycin tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 41 16809785
B complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 41 24448447
B complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 39 18322460
delta prp2 blocked spliceosome, S. cerevisiae, (Lardelli et al 2010) S. cerevisiae 38 20089683
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 36 19935684
Dre4 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 35 21386897
Prp19 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 35 21386897
C complex on PM5, H. sapiens, (Bessonov et al 2010) H. sapiens 32 20980672
B complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 32 24448447
C complex on MINX-GG, H. sapiens, (Bessonov et al 2010) H. sapiens 30 20980672
B complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 28 18981222
B complex via tobramycin tag, sample 1, H. sapiens, (Deckert et al 2006) H. sapiens 27 16809785
B complex sample 2, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 25 19941820
E complex IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 22 22110043
A complex, H. sapiens, (Behzadnia et al 2007) H. sapiens 20 17332742
U2 snRNP IP'd by antibody to PRPF40A, H. sapiens, (Marakov et al 2011) H. sapiens 20 22110043
B complex on Ftz, sample 2, H. sapiens, (Herold et al 2009) H. sapiens 19 18981222
28S B* complex, deltaPrp2 +Prp2/Spp2/ATP on M3Actin, 500 mM KCl wash, S. cerevisiae, (Warkocki et al 2009) S. cerevisiae 19 19935684
B complex on Zeste62, sample 3, D. melanogaster, (Herold et al 2009) D. melanogaster 17 18981222
B complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 16 18981222
P complex on AdML, short 3' exon, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 16 23345524
B complex on Zeste62, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 15 18981222
B complex on Zeste62, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 15 18981222
E complex IP'd by antibody to U1A, H. sapiens, (Marakov et al 2011) H. sapiens 15 22110043
C complex on ActinD31, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 14 19941820
Supraspliceosome, G. gallus, (Chen et al 2007) G. gallus 14 17537823
B complex on Ftz, sample 1, H. sapiens, (Herold et al 2009) H. sapiens 14 18981222
C complex on ActinACAC, S. cerevisiae, (Fabrizio et al 2009) S. cerevisiae 12 19941820
C complex on AdML, 3'SS mut, Velos, H. sapiens, (Ilagan et al 2013) H. sapiens 12 23345524
Mixed spliceosome complexes on AdML, H. sapiens, (Zhou et al 2002) H. sapiens 11 12226669
Mixed complexes Weri, H. sapiens, (Sharma et al 2008) H. sapiens 10 18193060
C complex sample 2, H. sapiens, (Bessonov et al 2008) H. sapiens 10 18322460
Saf2 tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 10 21386897
Supraspliceosome, H. sapiens, (Chen et al 2007) H. sapiens 9 17537823
A-like complex (A') HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 9 18193060
EDC-A complex Weri, H. sapiens, (Sharma et al 2008) H. sapiens 9 18193060
C complex on Ftz, sample 1, D. melanogaster, (Herold et al 2009) D. melanogaster 9 18981222
C complex on PM5, sample 2, H. sapiens, (Schmidt et al 2014) H. sapiens 9 24448447
C complex sample 1, H. sapiens, (Bessonov et al 2008) H. sapiens 7 18322460
penta-snRNP, S. cerevisiae, (Stevens et al 2002) S. cerevisiae 7 11804584
E-like complex (E') HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 7 18193060
B complex via MS2 tag, sample 2, H. sapiens, (Deckert et al 2006) H. sapiens 7 16809785
C complex on PM5, sample 1, H. sapiens, (Schmidt et al 2014) H. sapiens 7 24448447
C complex released in 1M NaCl, H. sapiens, (Bessonov et al 2008) H. sapiens 6 18322460
U2/U5/U6 complex Prp19-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 5 11884590
C complex, H. sapiens, (Jurica et al 2002) H. sapiens 5 11991638
EDC-A complex HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 5 18193060
Prp17tap-tag pulldown, S. pombe, (Ren et al 2011) S. pombe 5 21386897
EDC-E complex HeLa, H. sapiens, (Sharma et al 2008) H. sapiens 4 18193060
Mixed spliceosome complexes on Ftz, H. sapiens, (Zhou et al 2002) H. sapiens 4 12226669
C complex on Ftz, sample 2, D. melanogaster, (Herold et al 2009) D. melanogaster 3 18981222
U2/U5/U6 complex CDC5-tag, S. cerevisiae, (Ohi et al 2002) S. cerevisiae 2 11884590
U2/U5/U6 complex CDC5-tag, S. pombe, (Ohi et al 2002) S. pombe 2 11884590
E-like complex (E') Weri, H. sapiens, (Sharma et al 2008) H. sapiens 2 18193060
C complex 1M NaCl-washed core, H. sapiens, (Bessonov et al 2008) H. sapiens 1 18322460
H complex, H. sapiens, (Jurica et al 2002) H. sapiens 1 11991638
H complex, H. sapiens, (Sharma et al 2005) H. sapiens ■ 16109373
U2 17S, H. sapiens, (Will et al 2002) H. sapiens ■ 12234937
E complex Weri, H. sapiens, (Sharma et al 2005) H. sapiens ■ 16109373
EDC complex, H. sapiens, (Sharma et al 2005) H. sapiens ■ 16109373
B* complex, H. sapiens, (Makarov et al 2002) H. sapiens ■ 12411573
H complex Weri, H. sapiens, (Sharma et al 2005) H. sapiens ■ 16109373
Mixed spliceosome complexes, H. sapiens, (Neubauer et al 1999) H. sapiens ■ 9731529
Mixed spliceosome complexes, H. sapiens, (Rappsilber et al 2002) H. sapiens ■ 12176931
A complex, H. sapiens, (Hartmuth et al 2002) H. sapiens ■ 12477934
Prp1 tap-tag pulldown, S. pombe, (Carnahan et al 2005) S. pombe ■ 15755920
PSF-pulldown, without splicing substrate, H. sapiens, (Peng et al 2006) H. sapiens ■ 17114945
PSF-pulldown, with AdMl splicing subtrate, H. sapiens, (Peng et al 2006) H. sapiens ■ 17114945
B complex on MINX-MS2, ATPgammaS block, 40S glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562
A complex on MINX-MS2, ATPgammaS block, glycerol gradient peak, H. sapiens, (Agafonov et al 2016) H. sapiens ■ 27411562

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